Illumina Nextera Mate Pair Library Prep Kit

Illumina Nextera Mate Pair Library Prep Kit (12 indexes, 48 gel-free samples or 12 gel-plus samples)/FC-132-1001/1 Ea

產(chǎn)品編號(hào)： FC-132-1001

美  元  價(jià)： $4162.00

品       牌： Illumina

產(chǎn)       地： 美國

公       司： Illumina, Inc.

Product Highlights:

The Nextera Mate Pair Library Preparation Kit is an ideal approach for de novo sequencing, genome finishing, and the detection of structural variation. It is compatIBLe with large DNA genomes — even the most complex genomes such as those derived from cancer.

Sequence precious samples when limited DNA is available

Generate highly diverse libraries with premier data quality

Prepare sequencing-ready libraries in less than two days

Requires 10 Times Less DNA Input Than Other Protocols

Both gel-free and gel-plus protocols are available:

The gel-free protocol allows mate pair sequencing when limited DNA is available. It is designed for applications such as de novo assembly of small genomes.

The gel-plus protocol is ideal for challenging mate pair applications, such as de novo assembly of complex genomes.

Increased Library Diversity and Data Quality

The kit provides identifiable junction sequences for accurate identification of the two halves of the mate pair fragment.

Only biotinylates DNA molecules at fragmentation sites, avoiding troublesome internal biotinylation

Allows for the creation of millions of unique fragments

Increased library diversity generates fewer duplicate reads and premier data quality

Fast and Simple Mate Pair Workflow

The Nextera Mate Pair kit uses TruSeq DNA Library Preparation master-mixed reagents, thereby reducing the number of assay steps and hands-on time required.

Fewer pipetting steps simplifies workflow and reduces sample loss

Gel-free protocol option and on-bead reactions simplify purification steps and shorten protocol hands-on time

Specifications:

Assay Time 1.5 - 2 days

Hands-On Time 1.5 - 2 hours

Input Quantity 1 ug DNA for gel-free protocol, 4 ug DNA for gel-plus protocol

Multiplexing Can multiplex up to 12 samples, depending on genome size. CompatIBLe with most large DNA genomes.

Mechanism of Action Methods that target the junction site of large circularized fragments. Mechanical fragmentation (COVARIS).

System Compatibility MiSeq,HiSeq 3000,HiSeq 2500,HiSeq 4000

Species Category Other,Mammalian,Mouse,Human,Rat,Plant,Bacteria

Variant Class Single Nucleotide Polymorphisms (SNPs),Structural Variants,Insertions-Deletions (indels)

Method Whole-Genome Sequencing,De Novo Sequencing,Long-Read Sequencing

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